Composite

Part:BBa_K4613023:Design

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)


pET-46 Ek_LIC-ADH3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 827
    Illegal AgeI site found at 515
    Illegal AgeI site found at 677
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We attached 6*his tag to the N-terminus of ADH3 to get purified ADH3.


Source

NAU-CHINA 2023

References